Evaluation of some biochemical changes in diabetic patients

The present study consisted of 50 subjects were classified into three groups; Group [GI] Control group consisted of 10 clinically healthy adult subjects of both sexes free from any liver, kidney or cardiovascular diseases. Group [GII] diabetes mellitus type 1 consisted of 20 patients of both sexes. Group [GIII] diabetes mellitus type 1 with nephropathy consisted of 20 patients of both sexes. All subjects were undergo to the following investigated parameters; Ascorbic acid [vitamin C], Catalase, Total antioxidant capacity, Aldolase and Pyruvate kinase enzyme. vitamins C, catalase, total antioxidant capacity enzymes were highly significant decreased [P < 0. 01] in diabetes mellitus type 1 [GII] and diabetes mellitus type 1 with nephropathy [GIII] when compared to the control group. Adolase activity was highly significant increased [P < 0. 01] in diabetes mellitus type 1 with nephropathy [GIII] when compared to the control group. Pyruvate Kinase activity was highly significant increased [P < 0. 01] in diabetes mellitus type 1 [GII] when compared to the control group. The antioxidant and enzymes can be used for follow up in patients suffering from diabetes mellitus type 1 and predict other complications

Evaluation of the hydroxynitrile lyase activity in cell cultures of capulin (Prunus serotina) / 生物工程学报

Enzymatic preparations obtained from young plants and cell cultures of capulin were screened for hydroxynitrile lyase activity. The three week old plants, grown under sterile conditions, were used to establish a solid cell culture. Crude preparations obtained from this plant material were evaluated for the transformation of benzaldehyde to the corresponding cyanohydrin (mandelonitrile). The results show that the crude material from roots, stalks, and leaves of young plants and calli of roots, stalks, internodes and petioles biocatalyzed the addition of hydrogen cyanide (HCN) to benzaldehyde with a modest to excellent enantioselectivity.

Circulating cortisol & related steroids following ketoconazole challenge in Cushing's disease.

Circulating levels of cortisol, 17 alpha hydroxy progesterone and delta 4 androstenedione were analysed by specific radioimmunoassays in 11 patients with Cushing's disease before and at 2, 4, 6, 12 and 24# h following an oral dose of 400 mg ketoconazole. A significant fall in cortisol (26 - 79.6%, 59.8 +/- 18.7 SEM), delta 4 androstenedione (21.5 - 63.3%, 47.8 +/- 4.87) with a concomitant rise in 17 alpha hydroxy progesterone (113 - 218%, 116 +/- 11.43) were noted, suggesting inhibition of 17, 20 desmolase and 11 beta-hydroxylase enzymes in cortisol biosynthetic pathway.